Difference between revisions of "Part:BBa J45400:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
The coding regions (BAT2 and THI3) in this part came from S. cerevisae.
+
The coding regions (BAT2 and THI3) were PCRed out of the S. cerevisae genome.
  
 
===References===
 
===References===

Revision as of 04:59, 31 October 2006

3-methylbutanal generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Out of time constraints, we did not get rid of the EcoRI and SpeI cut sites in THI3. So...don't use EcoRI or SpeI when making assemblies with this part! IAGDactual.jpg

Source

The coding regions (BAT2 and THI3) were PCRed out of the S. cerevisae genome.

References