Difference between revisions of "Part:BBa K1413043:Experience"

(Applications of BBa_K1413043)
Line 4: Line 4:
  
 
===Applications of BBa_K1413043===
 
===Applications of BBa_K1413043===
This part is extract to plasmid pNK2 (see BBa_K1413044). This part is used to insert the transposon in genome of some bacteria like BL21 and Pseudovibrio denitrificans.  
+
This part is extract to plasmid pNK2 (see BBa_K1413044). This part is used to insert the transposon in genome of some bacteria like BL21 and Pseudovibrio denitrificans.
 +
 
 +
 
 +
<html>
 +
 
 +
<img src="https://static.igem.org/mediawiki/parts/c/ca/Kanamycine_gel_%281%29.png" width=50%/>
 +
 
 +
</html>
 +
 
 +
Picture of an electrophorese gel samples come from the PCR which amplify the kanamycineR cassette. All clones have the insertion of the transposon. The Control, Pseudovibrio denitrificans, do not the insertion as expected.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:16, 31 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1413043

This part is extract to plasmid pNK2 (see BBa_K1413044). This part is used to insert the transposon in genome of some bacteria like BL21 and Pseudovibrio denitrificans.


Picture of an electrophorese gel samples come from the PCR which amplify the kanamycineR cassette. All clones have the insertion of the transposon. The Control, Pseudovibrio denitrificans, do not the insertion as expected.

User Reviews

UNIQ42b6e7a752430bb4-partinfo-00000001-QINU UNIQ42b6e7a752430bb4-partinfo-00000002-QINU