Difference between revisions of "Part:BBa K1413002:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
This part have been engineered in order to improve the signal of GFP afer induced transcription.
 
This part have been engineered in order to improve the signal of GFP afer induced transcription.
−
It has been obtained by performing a mutation of 3 nucleotides within the sequence of RBS B0032.
+
It has been obtained by performing a mutation of 3 nucleotides within the sequence of RBS B0032.</br>
−
RBS B0032 = tca<b>cac</b>aggaaag
+
RBS B0032 = tca<b>cac</b>aggaaag  
−
 
+
New RBS (SD 001) = tca<b>agg</b>aggaaag</br>
−
New RBS (SD 001) = tca<b>agg</b>aggaaag
+
  
 
This novel RBS sequence has been designed in accordance with the consensus Shine-Dalgarno sequence: AGGAGGUAA and allows the RNA to bind more tightly to the 16S ribosome to initiate translation.
 
This novel RBS sequence has been designed in accordance with the consensus Shine-Dalgarno sequence: AGGAGGUAA and allows the RNA to bind more tightly to the 16S ribosome to initiate translation.

Revision as of 15:28, 31 October 2014


P0 promoter-RBS SD001-sfGFP- Terminator B0015 - Pr promoter-DmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1719
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1404
    Illegal BsaI.rc site found at 1945
    Illegal SapI.rc site found at 211
    Illegal SapI.rc site found at 2602


Design Notes

This part have been engineered in order to improve the signal of GFP afer induced transcription. It has been obtained by performing a mutation of 3 nucleotides within the sequence of RBS B0032.</br> RBS B0032 = tcacacaggaaag New RBS (SD 001) = tcaaggaggaaag</br>

This novel RBS sequence has been designed in accordance with the consensus Shine-Dalgarno sequence: AGGAGGUAA and allows the RNA to bind more tightly to the 16S ribosome to initiate translation.

Source

This part derives from BBa_K1413001.

References