Difference between revisions of "Part:BBa K1523008"

 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1523008 short</partinfo>
 
<partinfo>BBa_K1523008 short</partinfo>
  
 
It's a device which can reduce Cr(VI)
 
It's a device which can reduce Cr(VI)
 +
 +
The coding part of this device is K1523024, which is yieF gene from E.Coli K12. It can express the flavoprotein as a kind of chromate reductase.
 +
 +
Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr6+ producing Cr3+ and one electron to molecular oxygen without producing the toxic Cr5+ species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.
 +
 +
'''Catalytic activity'''
 +
 +
NAD(P)H + a quinone = NAD(P)+ + a hydroquinone.
 +
 +
2 NAD(P)H + Cr6+ + O2 = 2 NAD(P)+ + Cr3+ + H2O2.
 +
 +
'''Structure'''
 +
 +
[[File:Structure_yieF.jpg]]
 +
 +
This picture is from RCSB
 +
 +
'''Reference'''
 +
Uniprot : http://www.uniprot.org/uniprot/H0QEW2
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:02, 31 October 2014

A device can reduce Cr(VI)

It's a device which can reduce Cr(VI)

The coding part of this device is K1523024, which is yieF gene from E.Coli K12. It can express the flavoprotein as a kind of chromate reductase.

Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr6+ producing Cr3+ and one electron to molecular oxygen without producing the toxic Cr5+ species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.

Catalytic activity

NAD(P)H + a quinone = NAD(P)+ + a hydroquinone.

2 NAD(P)H + Cr6+ + O2 = 2 NAD(P)+ + Cr3+ + H2O2.

Structure

Structure yieF.jpg

This picture is from RCSB

Reference Uniprot : http://www.uniprot.org/uniprot/H0QEW2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]