Difference between revisions of "Part:BBa K1403017:Design"

 
 
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<partinfo>BBa_K1403017 short</partinfo>
 
<partinfo>BBa_K1403017 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Codon optimized using IDT's engine. The sRBS was generated by the Salis RBS calculator
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The purpose of this part is to biosynthesize acetoin using <i>E. coli</i>. The sequence was synthesized as a gBlock by IDT with the codon optimized <i>aldB</i> downstream of constitutive promoter <partinfo>BBa_J23108</partinfo> and a sRBS. Alpha-acetolactate decarboxylase transforms acetolactate to acetoin, which has a butter scent.
  
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===Source===
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Non-redundant <i>Lactococcus lactis</i> AldB from [http://www.ncbi.nlm.nih.gov/protein/500160484?report=genbank&log$=protalign&blast_rank=1&RID=46AXW03K01R NCBI: WP_011835154.1].
  
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===References===
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[https://parts.igem.org/Promoters/Catalog/Anderson Anderson Promoter Collection]
  
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===Source===
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David R. Nielsen, Sang-Hwal Yoon, Clara J. Yuan and Kristala L. J. Prather, Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli, January 2010.
  
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From L. lactis
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[https://salis.psu.edu/software/forward_withConstraints  Salis lab RBS calculator]<br>
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H.M Salis, Methods in Enzymology 2011<br>
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===References===
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H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009

Latest revision as of 07:39, 31 October 2014

Acetaldehyde dehydrogenase (aldB) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The purpose of this part is to biosynthesize acetoin using E. coli. The sequence was synthesized as a gBlock by IDT with the codon optimized aldB downstream of constitutive promoter BBa_J23108 and a sRBS. Alpha-acetolactate decarboxylase transforms acetolactate to acetoin, which has a butter scent.

Source

Non-redundant Lactococcus lactis AldB from [http://www.ncbi.nlm.nih.gov/protein/500160484?report=genbank&log$=protalign&blast_rank=1&RID=46AXW03K01R NCBI: WP_011835154.1].

References

Anderson Promoter Collection

David R. Nielsen, Sang-Hwal Yoon, Clara J. Yuan and Kristala L. J. Prather, Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli, January 2010.

Salis lab RBS calculator
H.M Salis, Methods in Enzymology 2011
H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009