Difference between revisions of "Part:BBa K1403003:Design"

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===Design Notes===
 
===Design Notes===
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The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.  
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The purpose of this part is to produce limonene in <i>E. coli</i>.
  
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We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later.
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We used LIMS1 CDS from part <partinfo>BBa_I742110</partinfo> and inserted constitutive promoter <partinfo>BBa_J23108</partinfo> and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.
  
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The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format.
+
The promoter and RBS were designed as oligos, aligned, digested, and ligated. The finished plasmid is in standard BioBrick format.
  
 
===Source===
 
===Source===
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<partinfo>BBa_I742110</partinfo>
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Registry. BBa_I742110
+
  
 
===References===
 
===References===
  
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Edinburg iGEM Project 2007
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[https://parts.igem.org/Part:BBa_I742110 iGEM 2007 Edinburgh]
 +
 
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[https://salis.psu.edu/software/forward Salis Lab RBS Calculator]<br>
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H.M Salis, Methods in Enzymology 2011<br>
 +
H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009

Latest revision as of 07:16, 31 October 2014

Limonene synthase (LIMS1) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

The purpose of this part is to produce limonene in E. coli.

We used LIMS1 CDS from part BBa_I742110 and inserted constitutive promoter BBa_J23108 and a sRBS upstream of the CDS. Limonene synthase 1 converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

The promoter and RBS were designed as oligos, aligned, digested, and ligated. The finished plasmid is in standard BioBrick format.

Source

BBa_I742110

References

iGEM 2007 Edinburgh

Salis Lab RBS Calculator
H.M Salis, Methods in Enzymology 2011
H.M. Salis, E.A. Mirsky, C.A. Voigt, Nat. Biotech., 2009