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===Usage and Biology===
 
===Usage and Biology===
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Revision as of 02:45, 31 October 2014

COI1

COI1 - COI1 is an E3 ligase that interacts with Jaz proteins (leading to their subsequent ubiquitination and degradation) after coronatin is introduced to the system. It is a system found in plants that is analogous to the auxin-TIR1-AID system studied by the Evry 2012 team.

The 2014 Nevada iGEM team cloned this part into a yeast integrating vector and observed its expression and functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1260
    Illegal BglII site found at 1590
    Illegal BamHI site found at 6
    Illegal XhoI site found at 967
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 826