Difference between revisions of "Part:BBa K1391112"
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<partinfo>BBa_K1391112 short</partinfo>
 
<partinfo>BBa_K1391112 short</partinfo>
  
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pEXPR_Tret:Coflin (Inactivated - pseudophosphorylated)
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Cofilin is an actin depolymerizing factor. It is recruited when PirB/LilrB2 is activated by beta-amyloid. [1]
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The 3rd amino acid in this construct has been mutated to make it pseudophophoralated and inactive. It was ordered from Adgene. In this construct, cofilin is under the inducible TreT promoter. It is activated in the presence of doxycyline.
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[1] Taeho Kim, George S. Vidal, Maya Djurisic, et al. "Human LilrB2 is a beta-amyloid recptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's Model". Science. 20 Sep 2013. <http://www.sciencemag.org/content/341/6152/1399>. 16 Oct 2014.
  
  

Revision as of 02:22, 31 October 2014

pEXPR_Tret:Cofilin (Inactivated - pseudophosphorylated)

Cofilin is an actin depolymerizing factor. It is recruited when PirB/LilrB2 is activated by beta-amyloid. [1]

The 3rd amino acid in this construct has been mutated to make it pseudophophoralated and inactive. It was ordered from Adgene. In this construct, cofilin is under the inducible TreT promoter. It is activated in the presence of doxycyline.


[1] Taeho Kim, George S. Vidal, Maya Djurisic, et al. "Human LilrB2 is a beta-amyloid recptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's Model". Science. 20 Sep 2013. <http://www.sciencemag.org/content/341/6152/1399>. 16 Oct 2014.


The characterization of the system this is part of can be found in the experience page.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 954
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
    Illegal NotI site found at 926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 954
    Illegal BglII site found at 12
    Illegal XhoI site found at 921
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
    Illegal NgoMIV site found at 1907
    Illegal NgoMIV site found at 3248
    Illegal NgoMIV site found at 3531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 1233
    Illegal BsaI.rc site found at 5056
    Illegal SapI site found at 1073
    Illegal SapI site found at 3976
    Illegal SapI.rc site found at 3097
    Illegal SapI.rc site found at 3307