Difference between revisions of "Part:BBa K1330000:Design"
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The PCR product was cut and ligated with pSB1C3.
 
The PCR product was cut and ligated with pSB1C3.
  
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The fusion with tRNA was designed as described in (tRNA article).
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The fusion with tRNA was designed as described in [http://www.ncbi.nlm.nih.gov/pubmed/17558412 Ponchon & Dardel].
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The Spinach2 sequence comes from (Spinach2 article). This BioBrick is a modified version of Spinach2 that has been modified (link to parts page) to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2.
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The Spinach2 sequence comes from [http://www.nature.com/nmeth/journal/v10/n12/full/nmeth.2701.html Strack ''et al.'']. This BioBrick is a modified version of Spinach2 that has been modified to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2. See [http://2014.igem.org/Team:DTU-Denmark/Achievements/Parts this page] for more details.
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===Source===
 
===Source===

Revision as of 22:56, 30 October 2014

Spinach2.1 flanked by tRNALys3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was synthesised, and amplified using High-Fidelity PCR. The PCR product was cut and ligated with pSB1C3.

The fusion with tRNA was designed as described in [http://www.ncbi.nlm.nih.gov/pubmed/17558412 Ponchon & Dardel]. The Spinach2 sequence comes from [http://www.nature.com/nmeth/journal/v10/n12/full/nmeth.2701.html Strack et al.]. This BioBrick is a modified version of Spinach2 that has been modified to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2. See [http://2014.igem.org/Team:DTU-Denmark/Achievements/Parts this page] for more details.

Source

References