Difference between revisions of "Part:BBa K1351022"

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[[File:Background_Fig.5.png|thumb|600px|center|Fig. 1. Predicted pairing between fsrA and the 5’-leader region of sdhC, the first gene of the sdhCAB operon.]]  
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 18:55, 30 October 2014

FsrA: Fur-regulated sRNA which binds the binding site sdhC

This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI: TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]


This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


Fig. 1. Predicted pairing between fsrA and the 5’-leader region of sdhC, the first gene of the sdhCAB operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]