Difference between revisions of "Part:BBa K1391133:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Some
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This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
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===Source===
 
===Source===

Revision as of 16:20, 30 October 2014


pEXPR_TRE:miRNAG4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3087
    Illegal NheI site found at 2124
    Illegal NheI site found at 2390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3087
    Illegal BamHI site found at 2554
    Illegal XhoI site found at 2984
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3087
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3609
    Illegal SapI.rc site found at 2001
    Illegal SapI.rc site found at 2602


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Artificial

References