Difference between revisions of "Part:BBa K1391105:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This gene is expressed under a hEF1a promoter for constitutive high level expression in mammalian cells. It adheres to RFC 65 for mammalian parts.
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This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
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(Ignore the RFC 65 link it is an automated link that links to a page unrelated to the iGEM RFC 65)
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===Source===
 
===Source===

Latest revision as of 16:15, 30 October 2014


pEXPR_hEF1a:CD79B-TCS-Gal4VP16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Human genome, viral genome, yeast genome

References