Difference between revisions of "Part:BBa K1351021"
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| − | [[File:LMU14_E1010mut-Pellet|thumb|right|the left eppendorf tube contains a pink bacterial pellet of ''E. coli'' containing the BioBrick BBa_K1351021 in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1351040 pBS0K-pPspac], a ''B. subtilis'' expression vector. On the right, you can see ''E. coli'' with the empty vector]] | + | [[File:LMU14_E1010mut-Pellet.jpg|thumb|right|the left eppendorf tube contains a pink bacterial pellet of ''E. coli'' containing the BioBrick BBa_K1351021 in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1351040 pBS0K-pPspac], a ''B. subtilis'' expression vector. On the right, you can see ''E. coli'' with the empty vector]] |
Revision as of 14:28, 29 October 2014
Monomeric Red Fluorescent Protein from Discosoma striata
Mutant of the Biobrick BBa_E1010 [[1]]. In order to make it compatible with the Freiburg-Standard RFC25, two AgeI-Restriction sites were deleted.
monomeric RFP: Red Fluorescent Protein.
Excitation peak: 584 nm Emission peak: 607 nm
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
| prefix with EcoRI, NotI, XbaI, SD and NgoMIV: | GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |
| suffix with AgeI, SpeI, NotI and PstI: | ACCGGTTAATACTAGTAGCGGCCGCTGCAG |
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]