Difference between revisions of "Part:BBa K1413041:Experience"
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===Applications of BBa_K1413041=== | ===Applications of BBa_K1413041=== | ||
| − | We used OriVR6Kgamma in the pNK2 ( | + | We used OriVR6Kgamma in the pNK2 (BBa_K141345). This plasmid contains a particular origin of replication OriVR6Kgamma. This oriVR6K gamma is controlled by the pi protein, which is encoded by the pir gene. Indeed, the pi protein allows the replication of the plasmid by binding a particular site in the ORI sequence. Hence, the oriVR6K gamma can only be replicated in a bacterial strain producing the pi protein. Also we used the cells DH5alpha pri, which replicated this plasmid. With other bacteria like BL21, or DH5alpha there wasn't replication. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 01:35, 29 October 2014
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Applications of BBa_K1413041
We used OriVR6Kgamma in the pNK2 (BBa_K141345). This plasmid contains a particular origin of replication OriVR6Kgamma. This oriVR6K gamma is controlled by the pi protein, which is encoded by the pir gene. Indeed, the pi protein allows the replication of the plasmid by binding a particular site in the ORI sequence. Hence, the oriVR6K gamma can only be replicated in a bacterial strain producing the pi protein. Also we used the cells DH5alpha pri, which replicated this plasmid. With other bacteria like BL21, or DH5alpha there wasn't replication.
User Reviews
UNIQf0bbe53705196b89-partinfo-00000000-QINU UNIQf0bbe53705196b89-partinfo-00000001-QINU