Difference between revisions of "Part:BBa K1440088:Experience"
Eric423627 (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K1440088=== | ===Applications of BBa_K1440088=== | ||
| + | |||
| + | This is testing result of ribozyme. In control group, we use plasmid contain mCherry alone to transfected HEK293T cell, and added a ribozyme at its 3’UTR region. Although the control group doesn’t emit much light itself, but the fact that the testing group report nearly no fluoresce signal is still a very astonishing result. This experiment demonstrates that the self-cleavage efficiency of this ribozyme is very high. | ||
| + | This ribozyme is derived from 3’UTR region of mouse CLEC2 gene, published in Nature 2008. According to the original paper, wild type ribozyme has a 241bp insert sequence between two functional arms, after delete this stuffer, the its efficiency boosts many folds. | ||
| + | The ribozyme we use here is also the one without insert sequence. This unexpected result of our experiment proved the high efficiency of this regulatory unit once again. | ||
| + | |||
| + | [[File:Ribozyme_efficiency.jpg|500px|thumb|left|alt text]] | ||
| + | |||
| + | [[File:Ribozyme_picture.jpg|300px|thumb|left|alt text]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 01:03, 29 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1440088
This is testing result of ribozyme. In control group, we use plasmid contain mCherry alone to transfected HEK293T cell, and added a ribozyme at its 3’UTR region. Although the control group doesn’t emit much light itself, but the fact that the testing group report nearly no fluoresce signal is still a very astonishing result. This experiment demonstrates that the self-cleavage efficiency of this ribozyme is very high. This ribozyme is derived from 3’UTR region of mouse CLEC2 gene, published in Nature 2008. According to the original paper, wild type ribozyme has a 241bp insert sequence between two functional arms, after delete this stuffer, the its efficiency boosts many folds. The ribozyme we use here is also the one without insert sequence. This unexpected result of our experiment proved the high efficiency of this regulatory unit once again.
User Reviews
UNIQ0c3a1532c7c27ff9-partinfo-00000000-QINU UNIQ0c3a1532c7c27ff9-partinfo-00000001-QINU