Difference between revisions of "Part:BBa J45992:Experience"
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J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was <bbpart>J45995</bbpart>. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (<bbpart>Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line). | J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was <bbpart>J45995</bbpart>. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (<bbpart>Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line). | ||
[[Image:BBa J45992-3characterization.png|600px]] | [[Image:BBa J45992-3characterization.png|600px]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 03:55, 31 October 2006
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Applications of BBa_J45992
J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was J45995. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (Q04401) and the signal was effectively turned off at stationary phase (green line).
User Reviews
UNIQbf4cf2276bbc3353-partinfo-00000002-QINU UNIQbf4cf2276bbc3353-partinfo-00000003-QINU