Difference between revisions of "Part:BBa K1330001"
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Spinach2 mutated with terminator | Spinach2 mutated with terminator | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | Spinach 2.1 RNA can bind and activate the fluorophore DFHBI, both in vivo and in vitro. This BioBrick also contains a terminator, so it can be placed after a promoter to measure its activity. The flanking tRNA comes from human tRNALys3 and increases stability and folding efficiency of the RNA, leading to higher fluorescent signal. It is possible to detect signal from Spinach2.1 when expressed from moderate strength promoters, e.g. the Anderson promoter library. | ||
| + | This part does not contain or need a Ribosome Binding Site. | ||
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Revision as of 20:59, 28 October 2014
Spinach2.1 flanked by tRNALys3 with terminator BBa_B0052
Spinach2 mutated with terminator
Usage and Biology
Spinach 2.1 RNA can bind and activate the fluorophore DFHBI, both in vivo and in vitro. This BioBrick also contains a terminator, so it can be placed after a promoter to measure its activity. The flanking tRNA comes from human tRNALys3 and increases stability and folding efficiency of the RNA, leading to higher fluorescent signal. It is possible to detect signal from Spinach2.1 when expressed from moderate strength promoters, e.g. the Anderson promoter library. This part does not contain or need a Ribosome Binding Site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]