Difference between revisions of "Part:BBa J45400:Design"

 
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<partinfo>BBa_J45400 short</partinfo>
 
<partinfo>BBa_J45400 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
Out of time constraints, we did not get rid of the EcoRI cut sites in THI3.  So...don't use EcoRI when making assemblies with this part!
+
Out of time constraints, we did not get rid of the EcoRI and SpeI cut sites in THI3.  So...don't use EcoRI or SpeI when making assemblies with this part!
  
  

Revision as of 03:48, 31 October 2006

3-methylbutanal generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Out of time constraints, we did not get rid of the EcoRI and SpeI cut sites in THI3. So...don't use EcoRI or SpeI when making assemblies with this part!


Source

The coding regions (BAT2 and THI3) in this part came from S. cerevisae.

References