Difference between revisions of "Part:BBa K1403002"

Revision as of 14:21, 28 October 2014

AgaA expression (3M2H-> 3H3MH)

AgaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It is an aminocylase that hydrolyzes 3-methyl-2-hexenoyl-glutamine (3M2H-gln) into 3-methyl-2-hexenoic acid (3M2H) and free glutamine. The substrate is secreted in sweat, and the product is a distinctive armpit odor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 288
Illegal BamHI site found at 519
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 181
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1403002 short</partinfo>
-agaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It metabolizes 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid, that is a sulphure volatile compound.
+AgaA is an enzyme present in <i>Corynebacterium striatum</i> that is the main responsible for body odor. It is an aminocylase that hydrolyzes 3-methyl-2-hexenoyl-glutamine (3M2H-gln) into 3-methyl-2-hexenoic acid (3M2H) and free glutamine. The substrate is secreted in sweat, and the product is a distinctive armpit odor.
-This part contains an agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Geneious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.
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 ===Usage and Biology===
+Body odor is a complex combination of odorants, but the major components come from just a few known enzymes. In the armpit, for example, a major souce of "pungent" or "musky" odors is the enzyme AgaA expressed by <i>Corynebacterium striatum </i> (Kligman <i>et al.</i>, 1981).
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