Difference between revisions of "Part:BBa K1431824"

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<partinfo>BBa_K1431824 short</partinfo>
 
<partinfo>BBa_K1431824 short</partinfo>
  
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Team Uppsala 2012 chromoprotein attracts many interests because its more convenient than fluorescent protein to be use as a reporter. However, few characterized data can be found for these proteins. SUSTC-Shenzhen this year want to tackle with theses problems.
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Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1431824 with chromoprotein amajLime (BBa_K1033916) this year and did characterization.
  
 
We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
 
We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
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'''For detailed characterization data, see the experience page.'''
  
 
===Selective Figures===
 
===Selective Figures===
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<center>https://static.igem.org/mediawiki/parts/6/62/31_916_BL21.jpg</center>
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<center>https://static.igem.org/mediawiki/parts/5/52/SUSTC-Shenzhen_Characterization_31_916_BL21.jpg</center>
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<center>'''LB agar plate of Biobricks BBa_K1431814 transformed in BL21 after incubating 23h at 37℃ (left)'''</center>
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<center>'''LB agar plate of Biobricks BBa_K1431824 transformed in BL21 after incubating 23h at 37℃ (left)'''</center>
  
  
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<center>https://static.igem.org/mediawiki/parts/3/3f/31_916_dh5.jpg</center>
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<center>https://static.igem.org/mediawiki/parts/5/51/SUSTC-Shenzhen_Characterization_31_916_DH5%CE%B1.jpg</center>
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<center>'''LB agar plate of Biobricks BBa_K1431814 transformed in DH5α after incubating 23h at 37℃ (left)'''</center>
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<center>'''LB agar plate of Biobricks BBa_K1431824 transformed in DH5α after incubating 23h at 37℃ (left)'''</center>
  
  
 
<center>https://static.igem.org/mediawiki/parts/9/95/SUSTC_31_916_L-4.jpg</center>
 
<center>https://static.igem.org/mediawiki/parts/9/95/SUSTC_31_916_L-4.jpg</center>
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<center>'''LB broth of Biobricks BBa_K1431814 after incubating 11h at 37℃,180rpm (left)'''</center>
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<center>'''LB broth of Biobricks BBa_K1431824 after incubating 11h at 37℃,180rpm (left)'''</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:29, 27 October 2014

amajLime, yellow-green chromoprotein reporter system (Weak Promoter, Weak RBS)

Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1431824 with chromoprotein amajLime (BBa_K1033916) this year and did characterization.

We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.

For detailed characterization data, see the experience page.

Selective Figures

SUSTC-Shenzhen_Characterization_31_916_BL21.jpg
LB agar plate of Biobricks BBa_K1431824 transformed in BL21 after incubating 23h at 37℃ (left)


SUSTC-Shenzhen_Characterization_31_916_DH5%CE%B1.jpg
LB agar plate of Biobricks BBa_K1431824 transformed in DH5α after incubating 23h at 37℃ (left)


SUSTC_31_916_L-4.jpg
LB broth of Biobricks BBa_K1431824 after incubating 11h at 37℃,180rpm (left)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]