Difference between revisions of "Part:BBa K1431814"

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We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
 
We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
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'''For detailed characterization data, see the experience page.'''
  
 
===Selective Figures===
 
===Selective Figures===

Revision as of 13:07, 27 October 2014

amajLime, yellow-green chromoprotein reporter system (Strong Promoter, Strong RBS)

Team Uppsala 2012 chromoprotein attracts many interests because its more convenient than fluorescent protein to be use as a reporter. However, few characterized data can be found for these proteins. SUSTC-Shenzhen this year want to tackle with theses problems.

We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.

For detailed characterization data, see the experience page.

Selective Figures

34_916_BL21.jpg
LB agar plate of Biobricks BBa_K1431814 transformed in BL21 after incubating 23h at 37℃(left)


34_916_dh5.jpg
LB agar plate of Biobricks BBa_K1431814 transformed in DH5α after incubating 23h at 37℃(left)


SUSTC_34_916-4.jpg
LB broth of Biobricks BBa_K1431814 after incubating 11h at 37℃,180rpm (left)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]