Difference between revisions of "Part:BBa K1351017"
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First results of this BioBrick are depicted in the following graph:
 
First results of this BioBrick are depicted in the following graph:
  
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[[File:LMU14 ComEdep promoters.png|600px]]
+
[[File:LMU14Sdpi.png|600px]]
  
  

Revision as of 09:23, 27 October 2014

SdpI with RBS: Immunity against the cannibalsim toxin sdpC of B. subtilis

This gene codes for the immunity against a cannibalism toxin SdpC of Bacillus subtilis. The sdpI gene is regulated by a separate promoter (PsdpIR) which is responsible for the operon sdpIR. Cannibalsim toxins are produced by B. subtilis mainly in mid to late stationary phase when nutrients are limited. The LMU-iGEM team also created an antisense mRNA BBa_K1351019 for the Bakillus module "suicide".

Prefix: TGCAGAATTCGCGGCCGCTTCTAGAGTAAGGAGGAACTACT
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


First results of this BioBrick are depicted in the following graph:

600px


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]