Difference between revisions of "Part:BBa K1489000:Experience"

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===Applications of BBa_K1489000===
 
===Applications of BBa_K1489000===
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Expression tests
 
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BBa_K148900 was transformed into chemically competent DH5-alpha cells and plated overnight with chloramphenicol challenge. One colony was incubated in 5 mL LB media + 34 ug/mL chloramphenicol and grown to mid-log phase at 37oC. Arabinose was added to a final concentration of 0.2% to initiate protein production and cells were re-incubated at 37 oC for 21 hours. Cells were then pelleted and lysed with 10% SDS solution. Cell lysate was centrifuged at 13200 rpm in a tabletop centrifuge for 1 hour to separate soluble and insoluble fractions. Both fractions were loaded on an SDS-PAGE gel for western blot analysis using anti-His antibodies.
 
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[[File:BovineGalectinExpression.jpg]]
 
  
 
===User Reviews===
 
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<partinfo>BBa_K1489000 AddReview number</partinfo>
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<partinfo>BBa_K1489000 1</partinfo>
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<I>Username</I>
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<I>Iowis Zhu</I>
 
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<I>Iowis Zhu</I>
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We have found that this biobrick can be functionally expressed in E. coli. This was confirmed via protein purification followed by quantification of a binding constant.
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[[File:Bovine_Binding_Assay.png]]
 
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Latest revision as of 06:22, 26 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1489000

User Reviews

UNIQ05f87750985cf005-partinfo-00000000-QINU

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Iowis Zhu

We have found that this biobrick can be functionally expressed in E. coli. This was confirmed via protein purification followed by quantification of a binding constant.

Bovine Binding Assay.png UNIQ05f87750985cf005-partinfo-00000002-QINU