Difference between revisions of "Part:BBa K1489001:Design"

 
 
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===References===
 
===References===
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Bourne Y, Bolgiano B, Liao DI, et al. Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides. Nat Struct Biol. 1994;1(12):863-70.

Latest revision as of 06:09, 26 October 2014

Bovine Galectin-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EcoRI site in middle of Bovine Galectin gene was removed, codons were optimized for expression in E. coli.


Source

Sequence generated from Bos taurus genome, reoptimized for E. coli expression.

References

Bourne Y, Bolgiano B, Liao DI, et al. Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides. Nat Struct Biol. 1994;1(12):863-70.