Difference between revisions of "Part:BBa K1371040:Design"

Revision as of 20:40, 7 October 2014 (view source) Registry (Talk | contribs)		Latest revision as of 05:32, 25 October 2014 (view source) Invly leung (Talk | contribs)
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	__NOTOC__		__NOTOC__
	<partinfo>BBa_K1371040 short</partinfo>		<partinfo>BBa_K1371040 short</partinfo>
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	===Design Notes===		===Design Notes===
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		+	== Candidate selection ==
		+	Biosynthesis of polyketides like erythromycin, tacrolimus and many others are guided by modular PKS genes. These genes encode large enzymes consisting of modules of domains, forming an "assembly line" to extend the ketide units to form polyketides. Among the known PKS genes, the genes coding for erythromycin producing polyketide synthethase have been extensively and deeply studied, and there are numerous information sources. Therefore, we chose a truncated erythromycin-producing polyketide synthase which has been proved to produce a simple triketide lactone 1B.<sup>[2]</sup><br/><br/>
	===Source===		===Source===
−	aa	+	<i>Saccharopolyspora erythraea</i>
	===References===		===References===
		+	[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.<p></p>
		+	[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, <i>E.coli</i> [J]. Science, 2001, 291(44): 1790-1792.<p></p>
		+	[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.<p></p>
		+	[4]Patrick Caffrey, Amphotericin biosynthesis in <i>Streptomyces</i> nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.<p></p>

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Latest revision as of 05:32, 25 October 2014

DEBS1(KR1)

Design Notes

Candidate selection

Biosynthesis of polyketides like erythromycin, tacrolimus and many others are guided by modular PKS genes. These genes encode large enzymes consisting of modules of domains, forming an "assembly line" to extend the ketide units to form polyketides. Among the known PKS genes, the genes coding for erythromycin producing polyketide synthethase have been extensively and deeply studied, and there are numerous information sources. Therefore, we chose a truncated erythromycin-producing polyketide synthase which has been proved to produce a simple triketide lactone 1B.^[2]

Source

Saccharopolyspora erythraea

References

[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.

[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, E.coli [J]. Science, 2001, 291(44): 1790-1792.

[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.

[4]Patrick Caffrey, Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.