Difference between revisions of "Part:BBa K1499401:Experience"
 
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To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499401 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity.
  
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The following gel image shows that PdomMRNAr1.2-08705.1 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. Pending cellulose-binding and waterproofing assays, C2 is a candidate for waterproofing both fungal mycelium and bacterial cellulose.
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[[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 1.</b> C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST.]]
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[[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 2.</b> C1 and C2 (PdomMRNAr1.2-08705.1) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that it this protein has functional chitin-binding domains as predicted by PSI-BLAST.]]
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===Applications of BBa_K1499401===
 
===Applications of BBa_K1499401===
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The protein encoded by this gene has shown potential chitin-binding activity. Pending assays for cellulose-binding activity and hydrophobicity, this protein could be used to waterproof chitin- and cellulose-based biomaterials – specifically in our case, fungal mycelium and bacterial cellulose.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 00:59, 25 October 2014

To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499401 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity.

Figure 1. C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST.

Applications of BBa_K1499401

The protein encoded by this gene has shown potential chitin-binding activity. Pending assays for cellulose-binding activity and hydrophobicity, this protein could be used to waterproof chitin- and cellulose-based biomaterials – specifically in our case, fungal mycelium and bacterial cellulose.

User Reviews

UNIQb2838257e5f4d4e1-partinfo-00000000-QINU UNIQb2838257e5f4d4e1-partinfo-00000001-QINU