Difference between revisions of "Part:BBa K1321113:Design"

(→‎Source)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
PhytoC+CBD10  with LacI
+
The fusion protein was cloned together using the NgoMIV and AgeI sites.
−
 
+
  
 +
The promotor was cloned onto the protein via the XbaI/SpeI sites.
  
 
===Source===
 
===Source===

Latest revision as of 20:18, 24 October 2014


Phytochelatin (PC) EC20 + linker-CBDcipA-linker with LacI promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 512
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 512
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 512
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 512
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part was made by inverse PCR of BBa_J04500, and ligated.

References