Difference between revisions of "Part:BBa K1321364:Design"
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===Source=== | ===Source=== | ||
| − | + | Our NiBP came from BBa_K1151001. | |
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| + | The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part. | ||
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| + | dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | ||
===References=== | ===References=== | ||
Latest revision as of 20:10, 24 October 2014
NiBP fused to dCBD with linker driven by T7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 232
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
The promotor was cloned onto the protein via the XbaI/SpeI sites.
Source
Our NiBP came from BBa_K1151001.
The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.
dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.