Difference between revisions of "Part:BBa K1321364:Design"

(Design Notes)
(Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
asdf
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Our NiBP came from BBa_K1151001.
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The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.
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dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.
  
 
===References===
 
===References===

Latest revision as of 20:10, 24 October 2014


NiBP fused to dCBD with linker driven by T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 232


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Our NiBP came from BBa_K1151001.

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References