Difference between revisions of "Part:BBa K1321362:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The fusion protein was cloned together using the NgoMIV and AgeI sites. | |
| − | + | ||
| + | The promotor was cloned onto the protein via the XbaI/SpeI sites. | ||
===Source=== | ===Source=== | ||
Latest revision as of 20:05, 24 October 2014
sfGFP fused to CBDcex driven by LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 245
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
The promotor was cloned onto the protein via the XbaI/SpeI sites.
Source
Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.
CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.
The LacI part came from BBa_J04500.