Difference between revisions of "Part:BBa K1321361:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The fusion protein was cloned together using the NgoMIV and AgeI sites. | |
| − | + | ||
| + | The promotor was cloned onto the protein via the XbaI/SpeI sites. | ||
===Source=== | ===Source=== | ||
| − | + | CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | |
| + | |||
| + | Our NiBP came from BBa_K1151001. | ||
| + | |||
| + | Our LacI promotor came from BBa_J04500. | ||
===References=== | ===References=== | ||
Latest revision as of 20:04, 24 October 2014
CBDcenA with linker fused to NiBP driven by LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
The promotor was cloned onto the protein via the XbaI/SpeI sites.
Source
CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.
Our NiBP came from BBa_K1151001.
Our LacI promotor came from BBa_J04500.