Difference between revisions of "Part:BBa K1499004"

Revision as of 18:47, 24 October 2014

Cellulose binding domains with streptavidin domain generator

This part encodes a protein with two cellulose-binding domains on either end joined by a streptavidin domain.

Usage and Biology

The idea behind this part is that each domain binds to a single cellulose fiber, thus providing a way to cross-link and strengthen cellulose polymers. In addition, the streptavidin domain allows for the modular addition of biotinylated proteins through protein-protein interactions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 703
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 304
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 651

Characterization

Verification of Part

The part was sequence verified before submission to the registry with two reads using VF2 and VR (Figure 1).

(figure here)

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1499004 short</partinfo>
-todo
+This part encodes a protein with two cellulose-binding domains on either end joined by a streptavidin domain.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The idea behind this part is that each domain binds to a single cellulose fiber, thus providing a way to cross-link and strengthen cellulose polymers. In addition, the streptavidin domain allows for the modular addition of biotinylated proteins through protein-protein interactions.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1499004 SequenceAndFeatures</partinfo>
+==Characterization==
+===Verification of Part===
+The part was sequence verified before submission to the registry with two reads using VF2 and VR (Figure 1).
+(figure here)