Difference between revisions of "Part:BBa K1371041"
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DEBS1 was fused with red fluorescent protein | DEBS1 was fused with red fluorescent protein | ||
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[[File:Pict11.png]] | [[File:Pict11.png]] | ||
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== Fluorescence detection analyses == | == Fluorescence detection analyses == | ||
To further confirm the expression of DEBS1, we monitored the fluorescence of RFP under Zeiss LSM710 confocal microscope (Fig.1). The results showed that after excitation with lazer source, red fluorescence was monitored in the RFP+DEBS1+TE transformant, while in the BL21 strain, no fluorescence was detected. These results indicate that RFP+DEBS1+TE was successfully expressed in the transformant. | To further confirm the expression of DEBS1, we monitored the fluorescence of RFP under Zeiss LSM710 confocal microscope (Fig.1). The results showed that after excitation with lazer source, red fluorescence was monitored in the RFP+DEBS1+TE transformant, while in the BL21 strain, no fluorescence was detected. These results indicate that RFP+DEBS1+TE was successfully expressed in the transformant. | ||
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<div class="center"> | <div class="center"> | ||
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Figure: Results of fluorescence detection | Figure: Results of fluorescence detection | ||
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Revision as of 17:01, 24 October 2014
RFP-DEBS1
DEBS1 was fused with red fluorescent protein
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2590
Illegal BamHI site found at 7135
Illegal BamHI site found at 9984 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1270
Illegal NgoMIV site found at 2753
Illegal NgoMIV site found at 3125
Illegal NgoMIV site found at 3158
Illegal NgoMIV site found at 3631
Illegal NgoMIV site found at 4340
Illegal NgoMIV site found at 5213
Illegal AgeI site found at 604
Illegal AgeI site found at 716
Illegal AgeI site found at 1081
Illegal AgeI site found at 2443
Illegal AgeI site found at 2789
Illegal AgeI site found at 3368
Illegal AgeI site found at 5089 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2195
Illegal BsaI.rc site found at 4625
Illegal SapI.rc site found at 3460
Fluorescence detection analyses
To further confirm the expression of DEBS1, we monitored the fluorescence of RFP under Zeiss LSM710 confocal microscope (Fig.1). The results showed that after excitation with lazer source, red fluorescence was monitored in the RFP+DEBS1+TE transformant, while in the BL21 strain, no fluorescence was detected. These results indicate that RFP+DEBS1+TE was successfully expressed in the transformant.
Figure: Results of fluorescence detection