Difference between revisions of "Part:BBa K1371041"
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== Fluorescence detection analyses ==
 
== Fluorescence detection analyses ==
 
To further confirm the expression of DEBS1, we monitored the fluorescence of RFP under Zeiss LSM710 confocal microscope (Fig.1). The results showed that after excitation with lazer source, red fluorescence was monitored in the RFP+DEBS1+TE transformant, while in the BL21 strain, no fluorescence was detected. These results indicate that RFP+DEBS1+TE was successfully expressed in the transformant.
 
To further confirm the expression of DEBS1, we monitored the fluorescence of RFP under Zeiss LSM710 confocal microscope (Fig.1). The results showed that after excitation with lazer source, red fluorescence was monitored in the RFP+DEBS1+TE transformant, while in the BL21 strain, no fluorescence was detected. These results indicate that RFP+DEBS1+TE was successfully expressed in the transformant.
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Figure: Results of fluorescence detection
 
Figure: Results of fluorescence detection
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1371041 parameters</partinfo>
 
<partinfo>BBa_K1371041 parameters</partinfo>
 
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Revision as of 17:01, 24 October 2014

RFP-DEBS1

DEBS1 was fused with red fluorescent protein

Pict11.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2590
    Illegal BamHI site found at 7135
    Illegal BamHI site found at 9984
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 2753
    Illegal NgoMIV site found at 3125
    Illegal NgoMIV site found at 3158
    Illegal NgoMIV site found at 3631
    Illegal NgoMIV site found at 4340
    Illegal NgoMIV site found at 5213
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
    Illegal AgeI site found at 1081
    Illegal AgeI site found at 2443
    Illegal AgeI site found at 2789
    Illegal AgeI site found at 3368
    Illegal AgeI site found at 5089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2195
    Illegal BsaI.rc site found at 4625
    Illegal SapI.rc site found at 3460


Fluorescence detection analyses

To further confirm the expression of DEBS1, we monitored the fluorescence of RFP under Zeiss LSM710 confocal microscope (Fig.1). The results showed that after excitation with lazer source, red fluorescence was monitored in the RFP+DEBS1+TE transformant, while in the BL21 strain, no fluorescence was detected. These results indicate that RFP+DEBS1+TE was successfully expressed in the transformant. Collage.jpg

Figure: Results of fluorescence detection