Difference between revisions of "Part:BBa K1371000:Design"
(2 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1371000 short</partinfo> | <partinfo>BBa_K1371000 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | |||
+ | == Candidate selection == | ||
+ | Biosynthesis of polyketides like erythromycin, tacrolimus and many others are guided by modular PKS genes. These genes encode large enzymes consisting of modules of domains, forming an "assembly line" to extend the ketide units to form polyketides. Among the known PKS genes, the genes coding for erythromycin producing polyketide synthethase have been extensively and deeply studied, and there are numerous information sources. Therefore, we chose a truncated erythromycin-producing polyketide synthase which has been proved to produce a simple triketide lactone 1B.<sup>[2]</sup><br/><br/> | ||
===Source=== | ===Source=== | ||
− | + | <i>Saccharopolyspora erythraea</i> | |
===References=== | ===References=== | ||
+ | [1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.<p></p> | ||
+ | [2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, <i>E.coli</i> [J]. Science, 2001, 291(44): 1790-1792.<p></p> | ||
+ | [3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.<p></p> | ||
+ | [4]Patrick Caffrey, Amphotericin biosynthesis in <i>Streptomyces</i> nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.<p></p> |
Latest revision as of 16:37, 24 October 2014
DEBS1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1878
Illegal BamHI site found at 6423
Illegal BamHI site found at 9273 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 558
Illegal NgoMIV site found at 2041
Illegal NgoMIV site found at 2413
Illegal NgoMIV site found at 2446
Illegal NgoMIV site found at 2919
Illegal NgoMIV site found at 3628
Illegal NgoMIV site found at 4501
Illegal AgeI site found at 369
Illegal AgeI site found at 1731
Illegal AgeI site found at 2077
Illegal AgeI site found at 2656
Illegal AgeI site found at 4377
Illegal AgeI site found at 4447
Illegal AgeI site found at 5074 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1483
Illegal BsaI.rc site found at 3913
Illegal SapI.rc site found at 2748
Design Notes
Candidate selection
Biosynthesis of polyketides like erythromycin, tacrolimus and many others are guided by modular PKS genes. These genes encode large enzymes consisting of modules of domains, forming an "assembly line" to extend the ketide units to form polyketides. Among the known PKS genes, the genes coding for erythromycin producing polyketide synthethase have been extensively and deeply studied, and there are numerous information sources. Therefore, we chose a truncated erythromycin-producing polyketide synthase which has been proved to produce a simple triketide lactone 1B.[2]
Source
Saccharopolyspora erythraea