Difference between revisions of "Part:BBa K1362423:Design"
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===Source=== | ===Source=== | ||
| − | This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[ | + | This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[105] |
===References=== | ===References=== | ||
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[1] [[2014.igem.org/Team:Heidelberg iGEM team Heidelberg 2014]] | [1] [[2014.igem.org/Team:Heidelberg iGEM team Heidelberg 2014]] | ||
| − | [2] [[link|RFC[ | + | [2] [[link|RFC[105]]] |
Latest revision as of 15:47, 24 October 2014
BsaI reverse restriction site for RFC[105] cloning
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2
Design Notes
This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[105] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
Source
This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [1]. All associated standard sequences can be reviewed in RFC[105]
References
[1] 2014.igem.org/Team:Heidelberg iGEM team Heidelberg 2014
[2] RFC[105]