Difference between revisions of "Part:BBa K1440066"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1440066 short</partinfo>
 
<partinfo>BBa_K1440066 short</partinfo>
  
  
−
this part consists of reverse CMV and reverse RFP and tet-on shRFP system. With the transfection of this plasmid into the cell lines, RFP will be expressed constitutely. if dox is dosed with the cell lines, shRFP will be expressed, then knockdown the RFP expression.It must be used in mammalian cells.
+
this part consists of reverse CMV and reverse RFP and tet-on shRFP system. With the transfection of this plasmid into the cell lines, RFP will be expressed constitutely. if dox is dosed with the cell lines, shRFP will be expressed, then knockdown the RFP expression.It must be used in mammalian cells.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:04, 24 October 2014

reverse CMV->reverse RFP->pTRE->shRNA


this part consists of reverse CMV and reverse RFP and tet-on shRFP system. With the transfection of this plasmid into the cell lines, RFP will be expressed constitutely. if dox is dosed with the cell lines, shRFP will be expressed, then knockdown the RFP expression.It must be used in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1184
    Illegal AgeI site found at 13
    Illegal AgeI site found at 125
    Illegal AgeI site found at 1116
  • 1000
    COMPATIBLE WITH RFC[1000]