Difference between revisions of "Part:BBa K1431814:Experience"

 
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__NOTOC__
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_K1431814===
 
===Applications of BBa_K1431814===
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After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431814 and BBa_K1431834 whose promoter is BBa_J23106, a relative weak promoter.
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==LB agar plate growth comparison==
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We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.
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JA is J23100,JB is J23106.
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At 00:30 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/b/bc/SUSTC-34_916-1.png</center>
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At 1:50  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/4/45/SUSTC-34_916-2.png</center>
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At 3:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/4/46/SUSTC-34_916-3.png</center>
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At 6:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/90/SUSTC_34_916-4.png</center>
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At 7:40  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/5e/SUSTC_34_916-5.png</center>
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At 8:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/7/7f/SUSTC_34_916-6.png</center>
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At 10:00 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/3/38/SUSTC_34_916-7.png</center>
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At 11:20 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/3/3d/SUSTC_34_916-8.png</center>
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==LB broth growth comparison==
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We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol.
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Then we isolated single colonies from the plates.
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At 00:50 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/2/24/SUSTC_34_916-1.png.jpg</center>
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At 02:45 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/59/SUSTC_34_916-2.png.jpg</center>
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At 04:55 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/7/78/SUSTC_34_916-3.jpg </center>
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At 10:40 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/c/c1/SUSTC_34_916-4.jpg </center>
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==Conclusion==
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'''With observation, BBa_K1431814 is weaker than BBa_K1431834 unexpectedly.'''
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 13:06, 23 October 2014

Applications of BBa_K1431814

After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431814 and BBa_K1431834 whose promoter is BBa_J23106, a relative weak promoter.

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

SUSTC-34_916-1.png


At 1:50 (16th,Oct.)

SUSTC-34_916-2.png


At 3:00 (16th,Oct.)

SUSTC-34_916-3.png


At 6:00 (16th,Oct.)

SUSTC_34_916-4.png


At 7:40 (16th,Oct.)

SUSTC_34_916-5.png


At 8:00 (16th,Oct.)

SUSTC_34_916-6.png


At 10:00 (16th,Oct.)

SUSTC_34_916-7.png


At 11:20 (16th,Oct.)

SUSTC_34_916-8.png

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

SUSTC_34_916-1.png.jpg


At 02:45 (17th,Oct.)

SUSTC_34_916-2.png.jpg


At 04:55 (17th,Oct.)

SUSTC_34_916-3.jpg


At 10:40 (17th,Oct.)

SUSTC_34_916-4.jpg

Conclusion

With observation, BBa_K1431814 is weaker than BBa_K1431834 unexpectedly.

User Reviews

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