Difference between revisions of "Part:BBa K1431813:Experience"

Latest revision as of 13:05, 23 October 2014

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

At 1:50 (16th,Oct.)

At 3:00 (16th,Oct.)

At 6:00 (16th,Oct.)

At 7:40 (16th,Oct.)

At 8:00 (16th,Oct.)

At 10:00 (16th,Oct.)

At 11:20 (16th,Oct.)

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates. ===BBa_K1431813 didn't grow on the plate, so single colonies for comparison was isolated.===

At 00:50 (17th,Oct.)

At 02:45 (17th,Oct.)

At 04:55 (17th,Oct.)

At 10:40 (17th,Oct.)

Conclusion

Unfortunately, BBa_K1431813 didn't grow and there isn't any color on plates or in broth!

User Reviews

UNIQb924cd5ce335c38d-partinfo-00000000-QINU UNIQb924cd5ce335c38d-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
-__NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
 ===Applications of BBa_K1431813===
-After we constructed this part, it was tranformed into BL21 & DH5α to test expression effeciency. In then meanwhile, we did a comparison between BBa_K1431812 and of which part promoter is changed to BBa_J23106(weak).
+After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431813 and another part with promoter BBa_J23106, a relatively weak promoter.
-We spread plate at 10:00 on 15th, Oct. Following are pictures which was taken every several hours after placing the plates into a incubator.
+==LB agar plate growth comparison==
+We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.
 JA is J23100,JB is J23106.
@@ Line 41: / Line 38: @@
 <center>https://static.igem.org/mediawiki/parts/c/c4/K11-8.png</center>
+==LB broth growth comparison==
-Then we isolated single colonies from the plates.
+We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol.
+Then we isolated single colonies from the plates. ===BBa_K1431813 didn't grow on the plate, so single colonies for comparison was isolated.===
 At 00:50 (17th,Oct.)
@@ Line 56: / Line 54: @@
-At 22:40 (17th,Oct.)
+At 10:40 (17th,Oct.)
 <center>https://static.igem.org/mediawiki/parts/4/4a/K11-4.jpg </center>
+==Conclusion==
 '''Unfortunately,  BBa_K1431813 didn't grow and there isn't any color on plates or in broth! '''<br>