Difference between revisions of "Part:BBa K1431813:Experience"

 
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__NOTOC__
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_K1431813===
 
===Applications of BBa_K1431813===
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After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431813 and another part with promoter BBa_J23106, a relatively weak promoter.
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==LB agar plate growth comparison==
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We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.
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JA is J23100,JB is J23106.
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At 00:30 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/9b/K11-1.png</center>
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At 1:50  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/2/22/K11-2.png</center>
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At 3:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/8/84/K11-3.png</center>
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At 6:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f8/K11-4.png</center>
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At 7:40  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/2/25/K11-5.png</center>
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At 8:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/c/cb/K11-6.png</center>
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At 10:00 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/d/dd/K11-7.png</center>
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At 11:20 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/c/c4/K11-8.png</center>
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==LB broth growth comparison==
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We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol.
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Then we isolated single colonies from the plates. ===BBa_K1431813 didn't grow on the plate, so single colonies for comparison was isolated.===
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At 00:50 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/52/K11-1.jpg</center>
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At 02:45 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f9/K11-2.jpg </center>
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At 04:55 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/3/3f/K11-3.jpg </center>
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At 10:40 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/4/4a/K11-4.jpg </center>
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==Conclusion==
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'''Unfortunately,  BBa_K1431813 didn't grow and there isn't any color on plates or in broth! '''<br>
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 13:05, 23 October 2014

Applications of BBa_K1431813

After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431813 and another part with promoter BBa_J23106, a relatively weak promoter.

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

K11-1.png


At 1:50 (16th,Oct.)

K11-2.png


At 3:00 (16th,Oct.)

K11-3.png

At 6:00 (16th,Oct.)

K11-4.png


At 7:40 (16th,Oct.)

K11-5.png


At 8:00 (16th,Oct.)

K11-6.png


At 10:00 (16th,Oct.)

K11-7.png


At 11:20 (16th,Oct.)

K11-8.png

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates. ===BBa_K1431813 didn't grow on the plate, so single colonies for comparison was isolated.===

At 00:50 (17th,Oct.)

K11-1.jpg


At 02:45 (17th,Oct.)

K11-2.jpg


At 04:55 (17th,Oct.)

K11-3.jpg


At 10:40 (17th,Oct.)

K11-4.jpg

Conclusion

Unfortunately, BBa_K1431813 didn't grow and there isn't any color on plates or in broth!

User Reviews

UNIQ408a52cb3e508d93-partinfo-00000000-QINU UNIQ408a52cb3e508d93-partinfo-00000001-QINU