Difference between revisions of "Part:BBa K1440000:Experience"
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'''===Applications of BBa_K1440000=== | '''===Applications of BBa_K1440000=== | ||
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This part originates from BBa_K812032 and BBa_K747096. This part is used to test the cre-TM system and pTRE system. It consists of loxp sites and pTRE promoter. In this part, although we use mCFP as the reporter gene, we find that the efficiency of mCFP is not quite good, so we replace the mCFP reporter gene with the EGFP to test the cre-TM system. And because it is a big part, so we also replace the mCFP with OSKM (four transcription factors) binding with T2A linkers, aiming to prove that using the pTRE promoter we found ourselves, we can successfully make fibroblast reprogram to iPSc. | This part originates from BBa_K812032 and BBa_K747096. This part is used to test the cre-TM system and pTRE system. It consists of loxp sites and pTRE promoter. In this part, although we use mCFP as the reporter gene, we find that the efficiency of mCFP is not quite good, so we replace the mCFP reporter gene with the EGFP to test the cre-TM system. And because it is a big part, so we also replace the mCFP with OSKM (four transcription factors) binding with T2A linkers, aiming to prove that using the pTRE promoter we found ourselves, we can successfully make fibroblast reprogram to iPSc. | ||
Revision as of 07:30, 23 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
===Applications of BBa_K1440000===
This part originates from BBa_K812032 and BBa_K747096. This part is used to test the cre-TM system and pTRE system. It consists of loxp sites and pTRE promoter. In this part, although we use mCFP as the reporter gene, we find that the efficiency of mCFP is not quite good, so we replace the mCFP reporter gene with the EGFP to test the cre-TM system. And because it is a big part, so we also replace the mCFP with OSKM (four transcription factors) binding with T2A linkers, aiming to prove that using the pTRE promoter we found ourselves, we can successfully make fibroblast reprogram to iPSc.
We will only show the result with EGFP and OSKM.
User Reviews
UNIQ99d061de494c0a42-partinfo-00000000-QINU UNIQ99d061de494c0a42-partinfo-00000001-QINU