Difference between revisions of "Part:BBa K1321335:Design"
(→Source) |
(→Source) |
||
| Line 11: | Line 11: | ||
Protein coding sequence from genome of Gluconacetobacter Xylinus ATCC 53582, available here: | Protein coding sequence from genome of Gluconacetobacter Xylinus ATCC 53582, available here: | ||
| + | <li>http://www.ncbi.nlm.nih.gov/nuccore/X54676.1 | ||
These parts were codon optimised for expression in <em>E. coli</em> and sent for gene synthesis | These parts were codon optimised for expression in <em>E. coli</em> and sent for gene synthesis | ||
===References=== | ===References=== | ||
Revision as of 07:11, 23 October 2014
acsCD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1837
Illegal BglII site found at 4382
Illegal BamHI site found at 1906 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 778
Illegal AgeI site found at 1334
Illegal AgeI site found at 1913
Illegal AgeI site found at 2123
Illegal AgeI site found at 2975
Illegal AgeI site found at 3293
Illegal AgeI site found at 3638 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequences of AcsC and AcsD were extracted from NCBI (refer to Source). Some native regulation was removed by codon-optimisation for expression in E.coli. The native ribosomal binding sites (RBS) were predicted to be weak by translation rate calculators (Sallis Lab RBS calculator and Postech UTR designer), therefore they were replaced by the B0034 strong RBS. Furthermore, the six base pairs flanking this RBS were edited with the Sallis Lab RBS calculator so as to tune RBS strength to try preserving the native stoichiometry.
Source
Protein coding sequence from genome of Gluconacetobacter Xylinus ATCC 53582, available here: