Difference between revisions of "Part:BBa J37015:Design"
(→‎Design Notes)
Line 9: Line 9:
 
*The part is designed to generate AHL via a positive feedback loop.
 
*The part is designed to generate AHL via a positive feedback loop.
 
*LuxR is constitutively produced by tetR, with LuxI under the control of Lux pR  
 
*LuxR is constitutively produced by tetR, with LuxI under the control of Lux pR  
−
*The part has been ligated from the following previous parts:
+
*The part has been ligated from the following previous parts: <bbpart>BBa_F2620</bbpart>,
−
::*<bbpart>BBa_F2620</bbpart>
+
<bbpart>BBa_C0261</bbpart>, <bbpart>BBa_I13504</bbpart>
−
::*<bbpart>BBa_C0261</bbpart>
+
*Expression of GFP downstream of the promoter serves as an indicator of the production of LuxI.
−
::*<bbpart>BBa_I13504</bbpart>
+
 
  
 
*More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Design here].
 
*More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Design here].

Revision as of 14:29, 30 October 2006

Prey Molecule Generator (AHL) + GFP Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1724
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2400


Design Notes

  • The part is designed to generate AHL via a positive feedback loop.
  • LuxR is constitutively produced by tetR, with LuxI under the control of Lux pR
  • The part has been ligated from the following previous parts: BBa_F2620,

BBa_C0261, BBa_I13504

  • Expression of GFP downstream of the promoter serves as an indicator of the production of LuxI.


  • More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Design here].

Source

N/A

References

  • Part F2620 has previously been studied by Barry Canton. More information about this receiver can be found [http://openwetware.org/wiki/Endy:F2620 here].