Difference between revisions of "Part:BBa K1429002"

Latest revision as of 00:28, 22 October 2014

Cyan Fluorescent Protein (CFP) "Cindy Lou" coding region, intellectual property-free

IP free synthetic non-Aequorea cyan fluorescent protein with ribosome binding site.

Can be used as a reporter. Production of CFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "CFP1":

The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra. Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1429002 short</partinfo>
-IP free cyan fluorescent protein.  We have a ribosome binding site (RBS).
+IP free synthetic non-Aequorea cyan fluorescent protein with ribosome binding site.
-Background
+===Usage and Biology===
+Can be used as a reporter. Production of CFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "CFP1":
-Making sure the inserts are here from the colony PCR results - are the GFP and RFP inserts present?
+https://static.igem.org/mediawiki/2014/b/bc/UV_light.jpg
+https://static.igem.org/mediawiki/2014/d/d7/20141015_174513.jpg
-Digest each plasmid with EcoRI/PstI. Incubate 15' at 37C.
-Run entire digest on 0.8% gel.
-Results
-These were slightly confounding. What I *think* happened is that the buffer wasn't optimal for one of the enzymes, so the digest wasn't run to completion. Why do I think this?
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
+The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1429002 SequenceAndFeatures</partinfo>