Difference between revisions of "Part:BBa K1429003"
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<partinfo>BBa_K1429003 short</partinfo>
 
<partinfo>BBa_K1429003 short</partinfo>
  
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"IP-free" orange fluorescent protein and ribosome binding site (RBS).
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IP free synthetic non-Aequorea orange fluorescent protein with ribosome binding site.  
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===Usage and Biology===
 
===Usage and Biology===
  
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Can be used as a reporter. Production of OFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "OFP2":
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https://static.igem.org/mediawiki/2014/b/bc/UV_light.jpg
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https://static.igem.org/mediawiki/2014/d/d7/20141015_174513.jpg
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The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1429003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1429003 SequenceAndFeatures</partinfo>

Latest revision as of 00:24, 22 October 2014

Orange Fluorescent Protein (OFP) "Yukon" coding region with RBS, intellectual property-free

IP free synthetic non-Aequorea orange fluorescent protein with ribosome binding site.


Usage and Biology

Can be used as a reporter. Production of OFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "OFP2":

UV_light.jpg

20141015_174513.jpg

The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2