Difference between revisions of "Part:BBa K1321365:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Codon optimised for E.coli. | |
| − | + | Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. | |
| + | The LacI vector was fused to the protein fusion using the Xba and SpeI site. | ||
===Source=== | ===Source=== | ||
| − | + | CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | |
| + | The LacI part came from BBa_J04500. | ||
===References=== | ===References=== | ||
Latest revision as of 23:40, 21 October 2014
CBDcenA with linker fused to sfGFP driven by LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
The LacI vector was fused to the protein fusion using the Xba and SpeI site.
Source
CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part came from BBa_J04500.