Difference between revisions of "Part:BBa K1321354:Design"
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===Source=== | ===Source=== | ||
− | + | The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part. | |
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+ | Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part. | ||
+ | |||
+ | CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. | ||
===References=== | ===References=== |
Revision as of 23:31, 21 October 2014
sfGFP fused to CBDclos driven by T7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 68
Design Notes
asdf
Source
The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.
Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.
CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.