Difference between revisions of "Part:BBa K1321150:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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GATC
+
Codon optimised for E.coli.
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+
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
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+
  
 
===Source===
 
===Source===

Revision as of 22:00, 21 October 2014


Metallothionein Fmt fused to linker-dCBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 204


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

GATC

References