Difference between revisions of "Part:BBa K1321120:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | Codon optimised for E.coli. | ||
+ | Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. | ||
===Source=== | ===Source=== | ||
− | + | Our SmtA was a modification of BBa_K19010, wherein we transformed it into Freiburg format. dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | |
===References=== | ===References=== |
Latest revision as of 21:29, 21 October 2014
SmtA metallothionein fused to linker-dCBD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
Source
Our SmtA was a modification of BBa_K19010, wherein we transformed it into Freiburg format. dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.