Difference between revisions of "Part:BBa K1321110:Design"
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===Source=== | ===Source=== | ||
| − | + | The LacI part was made by inverse PCR of BBa_J04500, and ligated. | |
===References=== | ===References=== | ||
Revision as of 20:14, 21 October 2014
Phytochelatin (PC) EC20 fused to linker-dCBD with LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.
Source
The LacI part was made by inverse PCR of BBa_J04500, and ligated.