Difference between revisions of "Part:BBa K1321104:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
CBD4+PhytoC
 
−
 
  
 +
Codon optimised for E.coli.
 +
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The T7 vector was fused to the protein fusion using the Xba and SpeI site.
  
 
===Source===
 
===Source===

Revision as of 19:44, 21 October 2014


CBDclos fused to Phytochelatin (PC) EC20 with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The T7 vector was fused to the protein fusion using the Xba and SpeI site.

Source

CBD4+PhytoC

References